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ls columns  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec ls columns
    Ls Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 8965 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ls+columns/LS+Columns/custom%40130-042-401%4042831169
    Average 99 stars, based on 8965 article reviews
    ls columns - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Senescent Fibroblasts Impair B Cell Proliferation via Cell–Cell Contact
    Article Snippet: .. PBMCs were thawed and naïve‐enriched B cells were isolated using the Naïve B Cell Isolation Kit II human (Miltenyi Biotec, #130‐091‐150) with LS columns (Miltenyi Biotec, #130‐042‐401), according to manufacturer's instructions. .. Purified naïve‐enriched B cells were adjusted to 10 6 cells/mL and labelled with 0.25 μM CTV (Invitrogen, # C34557 ) at 37°C for 20 min, according to manufacturer's instructions and cultured in complete (c)RMPI media containing 10% FBS, 2 mM L‐glutamine (Sigma‐Aldrich, #G7513), 1× penicillin–streptomycin (Sigma‐Aldrich, #P0781), and 50 μM 2‐Mercaptoethanol (Thermofisher, #31350‐010).

    Article Title: FDXR upregulation by p63/p73 is a prognostic and therapeutic marker of adult T-cell leukemia/lymphoma.
    Article Snippet: The homogenized lysate was then incubated with anti-TOM22 MicroBeads for 30 min at 4 °C. .. Mitochondria were purified by magnetic isolation using LS Columns (Miltenyi Biotec). .. Whole cells or isolated mitochondria were lysed in RIPA buffer complemented with cOmplete Protease Inhibitor Cocktail (Roche) for 5 min at 4 °C.

    Cell Isolation:

    Article Title: Senescent Fibroblasts Impair B Cell Proliferation via Cell–Cell Contact
    Article Snippet: .. PBMCs were thawed and naïve‐enriched B cells were isolated using the Naïve B Cell Isolation Kit II human (Miltenyi Biotec, #130‐091‐150) with LS columns (Miltenyi Biotec, #130‐042‐401), according to manufacturer's instructions. .. Purified naïve‐enriched B cells were adjusted to 10 6 cells/mL and labelled with 0.25 μM CTV (Invitrogen, # C34557 ) at 37°C for 20 min, according to manufacturer's instructions and cultured in complete (c)RMPI media containing 10% FBS, 2 mM L‐glutamine (Sigma‐Aldrich, #G7513), 1× penicillin–streptomycin (Sigma‐Aldrich, #P0781), and 50 μM 2‐Mercaptoethanol (Thermofisher, #31350‐010).

    Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells.
    Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 MS Columns Miltenyi Biotec Cat# 130-042-201 40 μm Cell Strainer Sarstedt Cat# 83.1826.001 24-well Cell Culture Plate Corning Cat# 3524 Storage: Prepare fresh immediately before use. ..

    Recombinant:

    Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells.
    Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 MS Columns Miltenyi Biotec Cat# 130-042-201 40 μm Cell Strainer Sarstedt Cat# 83.1826.001 24-well Cell Culture Plate Corning Cat# 3524 Storage: Prepare fresh immediately before use. ..

    Software:

    Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells.
    Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 MS Columns Miltenyi Biotec Cat# 130-042-201 40 μm Cell Strainer Sarstedt Cat# 83.1826.001 24-well Cell Culture Plate Corning Cat# 3524 Storage: Prepare fresh immediately before use. ..

    Magnetic Cell Separation:

    Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells.
    Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 MS Columns Miltenyi Biotec Cat# 130-042-201 40 μm Cell Strainer Sarstedt Cat# 83.1826.001 24-well Cell Culture Plate Corning Cat# 3524 Storage: Prepare fresh immediately before use. ..

    Mass Spectrometry:

    Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells.
    Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 MS Columns Miltenyi Biotec Cat# 130-042-201 40 μm Cell Strainer Sarstedt Cat# 83.1826.001 24-well Cell Culture Plate Corning Cat# 3524 Storage: Prepare fresh immediately before use. ..

    Cell Culture:

    Article Title: Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells.
    Article Snippet: .. Chemicals, peptides, and recombinant proteins Collagenase D Roche Cat# 11088882001 Dispase II Roche Cat# 04942078001 CaCl2 Thermo Fisher Scientific Cat# L13191.30 Trypan Blue Solution Gibco Cat# 15250061 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D4540 Dulbecco’s PBS (DPBS), without Ca2+ and Mg2+ Wisent Cat# 311-425-CL Critical commercial assays Adipose Progenitor Cell Isolation Kit (mouse) Miltenyi Biotec Cat# 130-106-639 Experimental models: Organisms/strains Mouse: Wild-type, C57BL/6J, male, 10–12 weeks old The Jackson Laboratory Stock No. 000664 Software and algorithms FlowJo v11 BD Biosciences https://www.flowjo.com GraphPad Prism v10 GraphPad Software https://www.graphpad.com Other DMEM/F-12 medium Gibco Cat# 12634010 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# 10082147 ACK Lysing Buffer Thermo Fisher Scientific Cat# J62150.AP L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin–streptomycin Wisent Cat# 450-201-ZL MACS Rinsing Buffer Miltenyi Biotec Cat# 130-091-222 MACS MultiStand Miltenyi Biotec Cat# 130-042-303 LS Columns Miltenyi Biotec Cat# 130-042-401 MS Columns Miltenyi Biotec Cat# 130-042-201 40 μm Cell Strainer Sarstedt Cat# 83.1826.001 24-well Cell Culture Plate Corning Cat# 3524 Storage: Prepare fresh immediately before use. ..

    Purification:

    Article Title: FDXR upregulation by p63/p73 is a prognostic and therapeutic marker of adult T-cell leukemia/lymphoma.
    Article Snippet: The homogenized lysate was then incubated with anti-TOM22 MicroBeads for 30 min at 4 °C. .. Mitochondria were purified by magnetic isolation using LS Columns (Miltenyi Biotec). .. Whole cells or isolated mitochondria were lysed in RIPA buffer complemented with cOmplete Protease Inhibitor Cocktail (Roche) for 5 min at 4 °C.



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